Ozone Therapy

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Ozone Therapy

Ozone Therapy

What is Ozone Therapy?

Ozone Therapy is the treatment of some diseases by using Medical Ozone gas (3-5% O3 - 95% O2).

Obtaining Medical Ozone

O3 gas; Devices called 'Medical Ozone Generator' are also obtained in an environment where a certain flow rate and 99.9%-100% pure medical oxygen is subjected to high electrical voltage. If normal air is used in ozone production, toxic N2 O2 (nitrogen dioxide) occurs due to the high nitrogen content, therefore it is not recommended.


All types of Ozone application can be successfully performed in our clinic:

Major Autohemotherapy (Patient's own blood ozonation and intravenous administration), Rectal Ozone application, Minor Autohemotherapy (Patients' own blood ozonation and intramuscular application) and Local Ozone gas application.


How Is Ozone Formed?

Ozone, which is in the stratosphere layer in the atmosphere, is formed on the one hand by the effect of ultraviolet radiation, and on the other hand is destroyed. This process occurs at different frequencies of ultraviolet radiation. 1 While the presence of ozone in the stratosphere is vital because it blocks ultraviolet B and C rays from the sun, its presence in the troposphere, the layer of air we live in, is very dangerous for the respiratory tract and is considered air pollution. 2 Ozone Therapy; It is the treatment of some diseases by using medical ozone gas (3-5% O3 - 95% O2) gas.


Mechanism of action of medical ozone

Medical ozone has well-known bactericidal (bactericidal), fungicidal (fungicide) and virostatic (inhibiting virus proliferation) properties. Therefore, it is used for disinfection of infected wounds and also for the treatment of diseases caused by bacteria and viruses.

Its ability to increase blood circulation is used in the treatment of circulatory disorders.

In low doses, it increases the body's resistance


We can do ozone application for the following ailments:

  • Arterial circulation disorders
  • immune regulation
  • Disorders after muscle trauma
  • rheumatoid arthritis
  • Knee, shoulder and hip joint arthrosis,
  • In the treatment of general infectious diseases (very effective against bacteria, fungi and viruses)
  • Wound cleansing effect
  • wound healing effect
  • Difficult-to-heal wounds
  • proctitis (inflammation of the breech)
  • Topical immune-activation
  • Colitis (in intestinal disorders)
  • revitalization (in Skin Care and as anti-Aging)
  • Widespread pain-Fibromyalgia syndrome
  • Waist – Neck Pain. In waist and neck hernias
  • In neuropathic pains-Diabetes-sugar nerve pains
  • In peripheral vascular diseases
  • As an aid in cancer treatment
  • In the prevention of circulatory disorders and vascular diseases
  • Reynaud's phenomenon (circulatory disorder in the hands)
  • Burger's disease (circulatory disorder in the feet)
  • Diabetes
  • metabolic syndrome
  • Colitis
  • rheumatic diseases
  • Chronic fatigue syndrome, fibromyalgia and stress
  • In the slowing of metabolism due to hypothyroidism
  • Sleeping disorders
  • Sudden hearing loss and tinnitus/dizziness
  • Supportive treatment for chemotherapy and radiotherapy in cancer
  • COPD (Chronic bronchitis) and Asthma and allergies
  • Yellow spot disease in the eye
  • Ensuring rapid recovery before and after surgery
  • burns
  • In the treatment of wounds after work accidents
  • lumbar hernia treatment
  • Diseases caused by viruses
  • Ozone therapy in vaginal bacterial and fungal infections


Application methods

  1. major autohemotherapy
  2. Minor Autohemotherapy
  3. external therapy
  4. Rectal or vaginal ozone application
  5. Intra-articular injection of ozone
  6. Intra-disc injection in neck and waist hernias and injection into the muscles around the spine


Medical Ozone Generators

Only 3% to 5% of this gas coming out of the generator consists of ozone, the rest is oxygen. O3 - Unstable, unstable molecule Since O3 is an unstable molecule, it enters chemical reactions more easily. It is more effective in creating a biological response. O3 is called active superoxygen because of its effects.


Liquids for Medical Purposes We Can Dissolve Ozone

O3 dissolves in liquids like other gases according to Henry's Law. Its dissolution depends on:

  • Plasma (Venous-Arterial)
  • Physiological saline (9% NaCl)
  • That
  • Vegetable oil (ideal olive oil) (Viebahn,1999)


Why Ozone Instead of Oxygen?

Oxygen is a more stable element than O3. The solubility of O3 in biological fluids is 10 times higher than that of oxygen. Therefore, the risk of embolism is less than oxygen. One of the most ideal solvents for O3 is oxygen.


Ozonoid Formation

Due to its unstability, O3 molecules react with each other or with other atom-molecules within seconds and turn into more stable O2 and OX (Ozonoid) molecules. O3 is no longer gaseous once it is applied to the body or any Medical Ozone Generator 0025 environment. In other words, there is no ozone gas in the body anymore.4,5 OX (Ozonoid) molecules turn into ROS and LOPs.


Free Radical Theory

Free Radicals (SR) are reactive substances that can be the initiator of various pathological processes, can be involved in intermediate steps, or can occur as a result. They can also occur in various physiological conditions such as respiratory explosion in mitochondria and phagocytes during aerobic respiration in the organism.


Oxidation Effect

O3 enters into oxidation reaction with all organic-inorganic compounds. It reacts with lipids (on the cell membrane, atherosclerotic plaque, etc.) and breaks the cell membrane, showing antimicrobial disinfection (it is a disinfectant 3125 times stronger than chlorine) on microorganisms (Herpes, HCV, HIV, Gr.Positive bacteria, etc.). It also shows anti-tumoral effect on tumor cell. It can neutralize toxins by oxidizing them.11,12 Bocci names ozone therapy as “Therapeutic Shock - Acute Oxidative Stress” because of some physiological and biochemical reactions. We can say that the essence of ozone therapy is "controlled oxidative stress" given to the body. 13 Tumor cells have “anaerobic metabolism”. When O3 gas is used directly, it oxidizes the outer lipid layer of malignant cells and lysis. Since tumor cells have a primitive life form, they have not been able to develop antioxidant systems (AOS) mechanisms that can protect themselves against oxidation. O3 therapy can be used in conjunction with conventional treatments for cancer.


For the last 20 years, the existence of “hypoxic cells” in human tumors has been indirectly proven. It is known that in experimental animal tumors, the presence of cells resistant to treatment - radiation - 0026 due to tissue hypoxia is known, and it is known that anemia in radiotherapy is one of the important bad prognostic factors. It has been shown that arterial oxygen content and hemoglobin levels are important prognostic factors in the evaluation of the tumor's response to radiation and are directly proportional to the growth delay in tumor tissue.14 O3 increases the radiochemobiological sensitizing effects by increasing tissue oxygenation and SR neutralization in the tissues. Tumor responses to chemotherapy (CT) have also been shown to contribute negatively to hypoxic cells. O3 therapy reduces side effects in cancer treatment; O3 therapy increases the leukocyte count. (reduces symptoms of bone marrow suppression due to chemotherapy and radiotherapy). O3 therapy prevents radiochemotherapy-induced fibrosis, vascular changes and polyneuropathies, activates AOS and reduces the SR and oxidative stress effects of chemotherapy and radiotherapy. O3 therapy increases energy production and improves performance-general condition. When O3 gas is applied directly on the tumor, it causes the cell membrane to break down with the oxidation effect. O3 therapy, eliminating hypoxia; It has an inhibitory effect on tumor angiogenesis. Also, O3 therapy activates the immune system.


Major Autohemotherapy (MAHT) as a Biological Effect Model

During MAHT, O3 dissolves rapidly in plasma. Some of the O3 is reduced by the antioxidants in the plasma. This sequence also occurs in ROS. The half-life of ROS is as short as seconds. Even before the blood is transfused back to the patient, these disappear and are replaced by oxidizing LOPs products such as H2 O2 formed by the oxyBagging Ozone Application Major Ozone Autohemotherapy Application Local Ozone Application Minor Autohemotherapy Application 0027 dation of the cell membranes.


Enzymatic-Noenzymatic Antioxidation System

a. Non-enzymatic AOS: Uric acid, ascorbic acid, protein (especially albumin), non-protein thiols, vitamin E and bilirubin.

b. Enzymatic AOS: From superoxide dismutase (SOD), catalase (CAT) to glutathione peroxidase (GPx) glutathione transferase (GST), glutathione (GSH) and glutathione reductase (GR).


Oxidation-Disinfectant Effect

Ozone has a strong oxidation-forming ability. Therefore, in the use of high concentration O3 gas LOCAL-EXTERNAL, Oxygen with OXIDATION is a more stable element than O3. The solubility of O3 in biological fluids is 10 times higher than that of oxygen. Therefore, the risk of embolism is less than oxygen. One of the most ideal solvents for O3 is oxygen. It is BACTERICIDAL, VIRICIDAL and FUNGIIDAL effective. Since cells of multicellular organisms have AOS, O3 does not have a damaging or irritating effect on tissues. Although ozone is an agent with a high disinfectant effect, ozone does not have such an effect in vivo.


Factors Affecting Dose

  • Systemic (Major Autohemotherapy, rectal, insufflation)
  • Loco-Systemic (Major and Minor blood application)
  • Local (such as cream, solution, bagging, sauna, etc.)
  • Combined Methods (The above methods are used together)



  • Therapeutic Doses
  • 1-80 mic/ml
  • Start with 5 mic/ml and work your way up to 30-40 mic/ml.



  • Low Dose: 1-20/30 Gamma
  • Medium Dose: 20/30-40/50 Gamma
  • High Dose: 40/50 Gamma and above


MAH – Serum Physiological (SF) Comparison


  • It has an antioxidant system.
  • It is a heterogeneous liquid.
  • It is mixed with less volume of gas.


Ozonized SF;

  • It does not have an antioxidant system.
  • A high percentage of peroxide is formed.
  • It is a homogeneous liquid.
  • Longer exposure to ozone with the fluid to be infused
  • there is a theme


Antioxidant System Dose

  • Activation Dose: 1-30 g/ml
  • Regulation Dose: 30-50 g/ml
  • Suppression Dose: 50-100 g/ml


Ozone-Dose-Frequency Relationship

Normally, ozone therapy is applied twice a week. However, it has been reported that it is done every day, even 3 times a day, and there is no problem. The volume of blood to be ozonated should not exceed 300 mL, especially in elderly and ill-constituted patients.